RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK + T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANKL is a 20.0 kDa polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues). Manufactured using all Animal-Free reagents.
- Carrier free
- Animal-free
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
Verified by N-terminal and Mass Spectrometry analyses (when applicable).
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:−20 °C
- Endotoxin Content:<0.1 ng/μg of protein (<1 EU/μg).
- Biological Activity:Determined by its ability to induce NF-κB in RAW264.7 cells in the absence of any cross-linking. The expectedED50 for this effect is 10.0-25.0 ng/ml.
- Animal-Free:Y
- Protein/Peptide Name:SRANK Ligand
- Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Formulation:Lyophilized
- Shipping Temperature:RT