Human Recombinant SCF (from E. coli)

About this item

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Verified by N-terminal and Mass Spectrometry analyses (when applicable).

Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.

Specifications

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological Activity:
The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.
Animal-Free:
Y
Protein/Peptide Name:
SCF
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping Temperature:
RT