- Antibody Type:Primary
- Antigen Name:Apoptotic Peptidase Activating Factor 1
- Antigen Symbol:APAF1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Epitope:N-Terminal
- Format:IgG
- Form:Liquid (sterile filtered)
- Gene ID:317
- Antigen Synonyms:DKFZp781B1145|CED4|KIAA0413
- Concentration:1 mg/mL by UV absorbance at 280 nm
- Shipping Temperature:Dry Ice
- Immunogen:Anti-Apaf1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
- Purification:Anti-Apaf1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Apaf1 from other sources has not been determined.
- Cat. No.:10800-050
- Supplier no.:600-401-Y26
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Recommended Dilutions: ELISA: 1:10,000; Immunohistochemsitry: 1 ?g/mL; Immunofluorescence Microscopy: 10 ?g/mL; Western Blot: 1 µg/mL; contains 0.02% (w/v) Sodium Azide
Type: Primary
Antigen: APAF1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: N-Terminal
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat