Specifications
- Antibody Type:Primary
- Antigen Name:Endonuclease G, mitochondrial
- Antigen Symbol:ENDOG
- Clonality:Monoclonal
- Clone:7G1G10
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Mouse
- Reactivity:Human,Rat
- Western Blot:Yes
- Size:100 µg
- Epitope:Center
- Format:IgG1
- Form:Liquid (sterile filtered)
- Gene ID:2021
- Antigen Synonyms:endonuclease G|mitochondrial endonuclease G|endo G|mitochondrial
- Concentration:1 mg/mL by UV absorbance at 280 nm
- Shipping Temperature:Dry Ice
- Immunogen:Anti-Recombinant protein corresponding Anti-EndoG antibody was produced in mice by repeated immunizations with recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
- Purification:Anti-EndoG Monoclonal Antibody was Protein A purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with EndoG [7G1G10] from other sources has not been determined.
- Cat. No.:10802-620
- Supplier no.:200-301-BA9
Specifications
About this item
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans . EndoG also cleaves DNA in vitro.
Recommended Dilutions: ELISA: 1:50,000-1:100,000; Western Blot: 5-10 ?g/mL; contains 0.02% (w/v) Sodium Azide
Type: Primary
Antigen: ENDOG
Clonality: Monoclonal
Clone: 7G1G10
Conjugation: Unconjugated
Epitope: Center
Host: Mouse
Isotype:
Reactivity: Human, Rat