CHOgro® Expression System, Mirus Bio

The CHOgro expression system is an optimized platform for transient, high titer protein production in suspension CHO derived cells. An animal-origin-free solution specifically formulated for optimal transfection-complex formation in conjunction with CHOgro™ Expression Media and TransIT-PRO™ Transfection Reagent.

• Efficient – Enables high protein titers with simple workflow
• Convenient – Quick adaptation to CHO cell lineages
• Optimized – High density growth with minimal cell clumping post transfection
• Worry-free – No commercial license required; animal origin free
• Optimized – Equimolar ratios of human IgG1 heavy & light chain constructs designed using DNA2.0 IP-Free vector technology
• Sensitive – Verify human IgG1 expression as a control using ELISA or other methods
• Compatible – Suitable for use with any large scale antibody expression system
• Ease of Use – Supplied at a ready-to-use 1.0 mg/ml concentration

CHOgro expression medium is a chemically defined, hydrolysate-free and animal-origin-free medium manufactured under cGMP conditions in an ISO-compliant facility. CHOgro is formulated to provide high density cell growth, and many suspension CHO cells (Freestyle™ CHO-S) can easily and quickly grow in CHOgro medium with minimal adaptation. TransIT-PRO® transfection reagent was developed by empirically testing proprietary lipid and polymer libraries for high transfection performance in suspension CHO and 293 cell types. It is free of animal-derived components and manufactured at Mirus Bio LLC in Madison, Wisconsin, USA.

The combination of CHOgro expression medium and TransIT® -PRO Transfection Reagent enables robust cell growth and high efficiency transfection that streamlines the transient protein expression process. With this platform, high yields of therapeutic candidates for preclinical studies are achieved.

This system consists of CHOgro expression medium and Poloxamer 188 medium supplements, TransIT-PRO® transfection reagent, and CHOgro complex formation solution. To verify expression a Huma IgG1 expression control is also available for purchase separately.

Poloxamer 188 is a non-ionic detergent that is added to batch mammalian cell cultures to reduce foaming and improve the resistance of cell membranes to hydrodynamic shearing.

 : Includes 2 L CHOgro expression medium, 1 ml TransIT-PRO® transfection reagent, 100 ml CHOgro complex formation solution, 100 ml Poloxamer 188 solution. All components can also be purchased separately. To verify expression, a Human IgG1 expression control is also available for purchase separately.