About this item
Biotin anti-human IFN-γ [4S.B3]; Isotype: Mouse IgG1, κ; Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus; Apps: ELISA Detection, ELISPOT Detection, ICFC; Size: 500 μg
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is 0.5 μg per 106 cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Type: Primary
Antigen: IFN-g
Clonality: Monoclonal
Clone: 4S.B3
Conjugation: Biotin
Epitope:
Host: Mouse
Isotype: Mouse IgG1, κ
Reactivity: Human;Cross-Reactivity: Chimpanzee;Baboon;Cynomolgus;Rhesus
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Specifications
- Catalog No:
- 502503-BL
- 502504-BL
- Antigen Symbol:
- IFN-g
- IFN-g
- Antigen Synonyms:
- Type II interferon|T cell interferon|Macrophage-activating factor (MAF)|IFNG|Interferon-γ|Immune interferon
- Type II interferon|T cell interferon|Macrophage-activating factor (MAF)|IFNG|Interferon-γ|Immune interferon
- Antibody Type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Conjugation:
- Biotin
- Biotin
- Clone:
- 4S.B3
- 4S.B3
- Reactivity:
- Baboon, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Human
- Baboon, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Human
- Host:
- Mouse
- Mouse
- Gene ID:
- 3458
- 3458
- Isotype:
- IgG1 kappa
- IgG1 kappa
- ELISA:
- Yes
- Yes
- Gel Shift:
- Yes
- Yes
- Flow Cytometry:
- Yes
- Yes
- Immunogen:
- Partially purified, native human IFN-γ
- Partially purified, native human IFN-γ
- Form:
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Purification:
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- Concentration:
- 0.50 mg/mL
- 0.50 mg/mL
- Storage Temperature:
- The antibody solution should be stored undiluted between 2 °C and 8 °C. Do not freeze.
- The antibody solution should be stored undiluted between 2 °C and 8 °C. Do not freeze.
- Shipping Temperature:
- Ambient RT
- Ambient RT