About this item
Measures Activated Caspases in intact cells by FACS
Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).
Species reactivity- Mammalian
Kit size- Convenient sizes (25 and 100 assays)
Applications- Detection of activated caspases in living cells.
Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
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Specifications
- Species Reactivity:Mammalian samples
- Shelf Life:6-12 months
- Sample Size:Live cells
- Storage Temperature:-20°C