- Antibody Type:Primary
- Antigen Name:beta-2-Microglobulin
- Antigen Symbol:B2M
- Clonality:Monoclonal
- Clone:B2M-01
- Conjugation:PE (Phycoerythrin)
- ELISA:Yes
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG2a
- Reactivity:Human
- Western Blot:Yes
- Size:100 μg
- Gene ID:567
- Storage Buffer:The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
- Storage Temperature:Store in the dark at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent.
- Immunogen:Purified human beta2-microglobulin
- Cat. No.:89295-866
- Supplier no.:GTX80228
PE-conjugated Mouse Monoclonal antibody [B2M-01] to beta2-microglobulin
Recommended Starting Dilutions:FACS: Use at 1 µg/mL. Positive Control: Peripheral blood lymphocytes. Negative control: DAUDI human Burkitt lymphoma cell line, erythrocytesIP: Note: The B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cellsWB: Use at 2-4 µg/mL. Positive Control: U937 human histiocytic lymphoma cell line RAJI human Burkitt lymphoma cell line. Negative control: EL4 mouse lymphoblastic lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE Sample buffer. Note: Both reducing and non-reducing conditionsIHC-P: Use at 10 µg/mL. Positive tissue: kidney glomeruliELISA: Note: Working dilution should be determinated by investigatorRIA: Note: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIAOptimal dilutions should be determined experimentally by the researcher.
Type: Primary
Antigen: B2M
Clonality: Monoclonal
Clone: B2M-01
Conjugation:
Epitope:
Host: Mouse
Isotype:
Reactivity: Human