Specifications
- Antibody Type:Primary
- Antigen Name:non-erythrocyte alpha-spectrin
- Antigen Symbol:non-erythrocyte alpha-spectrin
- Clonality:Monoclonal
- Clone:3D7
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1
- Reactivity:Pig,Human,Bovine,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Cross Adsorption:No
- Format:Lyophilized
- Antigen Synonyms:SPTAN1|non-erythroid alpha chain|SPTA2|Spectrin|alpha Fodrin|alpha II spectrin
- Immunogen:This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli.
- Cat. No.:10782-096
- Supplier no.:M-1575-100
Specifications
About this item
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. The Spectrins form into dimers and further into tetramers of alpha and beta subunits (Ref: Entrez Gene). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons.
Antibody recognizes spectrin in multiple species. Approved applications are: WB, IHC(IF). Fixation is 4% PFA or frozen tissues, not yet tested in paraffin embedded tissues. Recommended dilution of 1:500-1,000 for IHC/IF and 1:5,000-10,000 for WB. The protein is seen as a major band at 240kDa depending on the species.
Application Information:
WB, ICC and IHC. Recommended dilution of 1:1,000-1:2,000 for ICC and IHC, and 1:5,000-10,000 for WB. The protein is seen as a major band at 240 kDa depending on the species. Optimal concentrations/dilutions should be determined by the end-user.
Type: Primary
Antigen: non-erythrocyte alpha-spectrin
Clonality: Monoclonal
Clone: 3D7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Bovineine, Pig, Rat, Mouse