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Specifications
- Antibody Type:Primary
- Antigen Name:Internexin alpha
- Antigen Symbol:Internexin alpha
- Clonality:Monoclonal
- Clone:1D2
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1
- Reactivity:Mammals,Human,Rat,Mouse,Cat
- Western Blot:Yes
- Size:250 µL
- Cross Adsorption:No
- Format:Lyophilized
- Antigen Synonyms:NEF5|66 kDa neurofilament protein
- Immunogen:Recombinant rat alpha-internexin expressed and purified from E. coli
- Cat. No.:10782-064
- Supplier no.:M-1378-250
Specifications
About this item
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species.
Application Information:
Western Blotting (WB), Immunocytochemistry (IC) and Immunohistochemistry on paraffin sections (IHC). The recommended dilution for WB is 1:5000-10,000. The epitope recognised by this antibody is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation so this antibody is ideally suited for studies of paraffin embedded formalin fixed histological sections. The recommended dilution for IHC is 1:1,000-5,000. For IC, dilution is 1:250-500. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Internexin alpha
Clonality: Monoclonal
Clone: 1D2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Rat, Mouse, Cat, Mammals