Human Recombinant TNF-A (from E. coli)

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS).

High quality
Low endotoxin
Affordable

TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

: For research use only.

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Conjugation:
Unconjugated
Protein Function:
Cytokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
Endotoxin LAL (EU/µg) ≤1
Biological Activity:
Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5.0×10⁵ units/mg
Gene ID:
P01375
Reconstitution Instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-Free:
Y
Protease-free:
N
Animal-Free:
N
Protein Synonyms:
DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin
UniProtKB:
P01375
Protein/Peptide Name:
TNF-A
Purity:
≥95%
Molecular Weight:
17.5 kDa
Sequence:
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Endotoxin Level:
Low
Formulation:
10 mM sodium phosphate, pH 7.5
Nuclease-free:
N
Grade:
RUO
Shipping Temperature:
RT
Tested Applications:
Bioassay

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