Sensitive probe for the measurement of intracellular oxygen concentration levels

The Mito-ID® Intracellular O2 Probe is a metalloporphyrin based oxygen-sensitive probe developed for the intracellular analysis of molecular oxygen using plate-based, time-resolved fluorometry. The assay is based on the ability of oxygen to quench the excited state of the oxygen-sensitive reagent. The probe is taken up via non-specific energy dependent endocytosis and, after washing, the cells are monitored on a TR-F plate reader. Probe phosphorescence is quenched by intracellular oxygen in a non-chemical, reversible manner allowing the measurement of average intracellular oxygen levels and facilitating real-time monitoring of relative changes in cellular oxygen concentration.

Probe signal increases with a reduction in intracellular oxygen and deceases with an increase in intracellular oxygen. Probe signal can be converted to absolute oxygen concentrations following calibration of probe. The flexible plate reader format, allows multiparametric or multiplex combination with a range of other reagents.