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Specifications
- Assay Type (ELISA with LOV):Competitive
- Host:
- Primary antibody reactivity:
- Target protein:cGMP
- Environmentally Preferable:
- Sensitivity:Assay Buffer : 0.42 pmol/mL (non-acetylated) 0.043 pmol/mL (acetylated) HCl: 0.604 pmol/mL (non-acetylated) 0.059 pmol/mL (acetylated) (range 0.8 - 500 pmol/mL (non-acetylated); 0.08 - 50 pmol/mL (acetylated))
- Cat. No.:89141-342
- Supplier no.:901-164
- No. of tests:96 wells
Specifications
About this item
Most sensitive (0.043 pmol/mL) and complete colorimetric kit for intra- and extracellular cGMP quantification.
For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.