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Q5® Site-Directed Mutagenesis Kit (Without Competent Cells), New England Biolabs
Q5® Site-Directed Mutagenesis Kit (Without Competent Cells), New England Biolabs
Catalog # 102877-562
Small Business Enterprise
CAS Number:  
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Q5® Site-Directed Mutagenesis Kit (Without Competent Cells), New England Biolabs
Catalog # 102877-562
Supplier Number:  E0552S
Small Business Enterprise
CAS Number:  

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Specifications

  • Description:
    Q5 Site-Directed Mutagenesis Kit (Without Competent Cells)
  • Size:
    10 Reactions
  • Cat. No.:
    102877-562
  • Supplier no.:
    E0552S

Specifications

About this item

The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours

The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, not supplied, ensures robust results with plasmids up to at least 20 kb in length.