Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin. New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

• Sarcomeric myosin positive
• Available from different muscles
• Differentiation capacity to multinucleate syncytia
• Growth and Differentiation Media available

SkMC are optimal for in vitro muscle studies

This product can be used in the following applications. Gene therapy research, Transplantation research, Developmental / differentiation studies, Tissue repair research, Pharmacological testing, Neuromuscular disease research.

They proliferate very well in the mitogen-rich PromoCell Skeletal Muscle Cell Growth Medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using the PromoCell Skeletal Muscle Cell Differentiation Medium.