One unit will oxidize 1.0 µmole of L-ascorbate to dehydroascorbate per minute at pH 5.6 and 20 °C.
Substrate Specificity: The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives.
Stabilizers: Bovine serum albumin (BSA), borax, basic amino acids. Michaelis Constant: 2.5 x 10^-4 M (Ascorbate). Inhibitors: Azide, cyanide, Na2S, diethyldithiocarbamate (Na). Optimum Temperature: Approx. 30 °C. Thermal Stability: Below 40 °C (pH 8.0, 30 min).
Ascorbate Oxidase is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
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- CAS No.:9029-44-1
- Form:Lyophilized powder
- Enzyme Name:Ascorbate Oxidase