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Human Recombinant Epidermal Growth Factor (from E. coli), MP Biomedicals
Human Recombinant Epidermal Growth Factor (from E. coli), MP Biomedicals
Catalog # IC15457180
Supplier:  MP Biomedicals
CAS Number:  
Human Recombinant Epidermal Growth Factor (from E. coli), MP Biomedicals
Catalog # IC15457180
Supplier:  MP Biomedicals
Supplier Number:  0215457180
CAS Number:  

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Specifications

  • CAS No.:
    62229-50-9
  • Protein/Peptide Type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Size:
    100 µg
  • Form:
    White Lyophilized Powder
  • Protein Synonyms:
    EGF|EGF human
  • Protein/Peptide Name:
    Epidermal Growth Factor
  • Purity:
    >98%
  • Formulation:
    Sterile filtered and then the protein was lyophilized with no additives.
  • Cat. No.:
    IC15457180
  • Supplier no.:
    15457180

Specifications

About this item

Soluble in water and most aqueous buffers. For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL.

Expressed in: E. coli, Endotoxin level is less than 0.1 ng per mg (1EU/mg), Biological Activity: Human EGF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <0.1ng/mL, corresponding to a specific activity of >1 x 10^7 units/mg.

Epidermal Growth Factor, Human, Recombinant is a recombinant analog of human epidermal growth factor (EGF) that provides a potent and cost-effective mitogen for cell culture applications. It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. It is effective in a variety of fibroblast and endothelial cell types and produces extremely potent results in cell culture. It is produced in a patented E. coli expression system free of animal-derived components.

The lyophilized protein should be reconstituted in water to a concentration of 1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.