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Specifications
- Size:25 U
- Form:Powder
- Enzyme Name:Pyruvate Oxidase
- Cat. No.:IC15348625
- Supplier no.:15348625
Specifications
About this item
One unit causes the formation of one µmole of hydrogen peroxide (half a µmole of quinoneimine dye) per minute at pH 5.7 at 37 °C.
Michaelis constant : 3.4 X 10^-4M (Pyruvate) Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++ Optimum temperature : 65°C, pH Stability : pH 5.7 - 6.5 (25°C,, 20hr) Thermal stability : below 45°C, (pH 6.0, 15min)
Pyruvate Oxidase reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.