Collagenases degrade native helical collagen fibrils. The enzyme has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. It is a type II enzyme that contains greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells. Ca2+ is required for its activation while metal chelating agents such as cysteine, EDTA or o-phenanthroline inhibits its activity.
- Presentation: Brown Lyophilized Powder
- pH: 7-9 for the A-a enzyme (optimum pH)
- Soluble in water
Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.
Collagenase used for collagen structural and biosynthetic studies
For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.
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- CAS No.:9001-12-1
- Source:Clostridium histolyticum
- Species:Clostridium histolyticum
- Storage Conditions:Store at +4 °C. Store Desiccated
- Form:Brown Lyophilized Powder
- MDL No.:MFCD00130830
- Enzyme Name:Collagenase Type II
- Enzyme Activity:≥125 U/mg solid
- Solubility:Soluble in water