Specifications
- Antibody Type:Primary
- Antigen Symbol:Biotin
- Clonality:Polyclonal
- Conjugation:TRITC (Tetramethylrhodamine Isothiocyanate)
- ELISA:Yes
- Flow Cytometry:Yes
- Host:Goat
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG
- Western Blot:Yes
- Size:1.0 mg
- Cross Adsorption:No
- Format:Purified IgG
- Form:Lyophilized
- Storage Buffer:10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
- Storage Temperature:2-8 °C
- Concentration:1.0 mg/ml (E 1% at 280 nm = 13.0)
- Shipping Temperature:2-8 °C
- Immunogen:BSA-Biotin, KLH-Biotin
- Tested Applications:Immunomicroscopy
- Purification:Affinity purified using solid phase biotin
- Cat. No.:10149-526
- Supplier no.:IR1362
Specifications
About this item
TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555nm / 580nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)
- Origin: US
ImmunoReagents' antibodies are suitable for most immunoassay formats and purified, cross adsorbed and conjugated based on the final product specfications.
Products will be accompanied with a standard product CoA to include application, purity and cross reactivity profiles.
Type: Primary
Antigen: BSA-Biotin, KLH-Biotin
Clonality: Polyclonal
Clone:
Conjugation: TRITC (Tetramethylrhodamine Isothiocyanate)
Epitope:
Host: Goat
Isotype: IgG
Reactivity: 0