The One Step RT-PCR Master Mix Kit allows for rapid analysis of gene expression from tissues and cells

The rTth DNA polymerase is provided in a 2X master mix with an antibody or antibody-mediated hot start, optimized buffer and ultrapure deoxynucleotides. The antibody-mediated hot start enhances specificity of both reverse transcription and PCR. This kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction.

The kit can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridization, dot blots, S nuclease assays and conventional two-step RT-PCR. The Once Step RT-PCR Master Mix kit utilizes recombinant Thermus thermophilus (rTth) DNA polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent RNA polymerase.