The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.
Type: Primary
Antigen: CD8A
Clonality: Monoclonal
Clone: RPA-T8
Conjugation: APC (Allophycocyanin)
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity:
- Catalog No:
- 10050-444
- 10050-440
- 10050-442
- Antigen Symbol:
- CD8A
- CD8A
- CD8A
- Antigen Name:
- CD8 antigen, alpha chain
- CD8 antigen, alpha chain
- CD8 antigen, alpha chain
- Antigen Synonyms:
- p32|Lyt-66|BB154331|Ly-2|Leu2|Ly-35|MAL|CD8|Ly-B
- p32|Lyt-66|BB154331|Ly-2|Leu2|Ly-35|MAL|CD8|Ly-B
- p32|Lyt-66|BB154331|Ly-2|Leu2|Ly-35|MAL|CD8|Ly-B
- Antibody Type:
- Primary
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Monoclonal
- Conjugation:
- APC (Allophycocyanin)
- APC (Allophycocyanin)
- APC (Allophycocyanin)
- Clone:
- RPA-T8
- RPA-T8
- RPA-T8
- Reactivity:
- Baboon, Cynomolgus monkey, Sooty mangabey, Macaque, Chimpanzee, Human
- Baboon, Cynomolgus monkey, Sooty mangabey, Macaque, Chimpanzee, Human
- Baboon, Cynomolgus monkey, Sooty mangabey, Macaque, Chimpanzee, Human
- Host:
- Mouse
- Mouse
- Mouse
- Gene ID:
- 925
- 925
- 925
- Isotype:
- IgG1
- IgG1
- IgG1
- Flow Cytometry:
- Yes
- Yes
- Yes
- Neutralisation:
- Yes
- Yes
- Yes
- Storage Temperature:
- Store Undiluted at 2 to 8°C; Protect from Light; Do Not Freeze.
- Store Undiluted at 2 to 8°C; Protect from Light; Do Not Freeze.
- Store Undiluted at 2 to 8°C; Protect from Light; Do Not Freeze.
- Tested Applications:
- Flow Cytometry
- Flow Cytometry
- Flow Cytometry