Specifications
- Protein/Peptide Type:Recombinant
- Source:CHO cells
- Species:Human
- Size:0.01 mg
- Biological Activity:Biological activity was determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5-0.7 μg/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
- Protein Synonyms:Basement-membrane protein 40|ON|SPARC|BM-40
- Protein/Peptide Name:Osteonectin
- Purity:>98%
- Formulation:Lyophilized
- Cat. No.:10073-048
- Supplier no.:40-571
Specifications
About this item
SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation.
Store in working aliquots at -20°C. Avoid freeze/thaw cycles.