Heart Lysate (7 day old mouse), ProSci
Catalog # 10062-376
Supplier: Prosci
CAS Number:
Specifications
- Cell Format:Cryopreserved
- Description:Normal
- Size:0.1 mg
- Storage Conditions:Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Storage Buffer:Heart lysate (7 day old mouse) is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Concentration:2 mg/mL
- Tested Applications:WB
- Cat. No.:10062-376
- Source:Mouse
- Tissue:Heart
- Supplier no.:1401-7
Specifications
About this item
Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.