Duodenum Tumor Lysate, ProSci Inc
Catalog # 10062-158
Supplier: Prosci
CAS Number:
Specifications
- Cell Format:Cryopreserved
- Description:Tumor
- Size:0.1 mg
- Storage Conditions:Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Storage Buffer:Duodenum Tumor lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Concentration:2 mg/mL
- Tested Applications:WB
- Cat. No.:10062-158
- Source:Human
- Tissue:Duodenum
- Supplier no.:1328
Specifications
About this item
Duodenum tumor tissue lysate was prepared by homogenization in lysis buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS)
Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.