Specifications
- Antibody Type:Primary
- Antigen Name:dynamin 1
- Antigen Symbol:Dnm1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Sheep
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:0.1 mL
- Form:Liquid
- Gene ID:P21575
- Antigen Synonyms:Dnm1
- Modification:Ser 774
- Storage Buffer:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
- Molecular Weight:95
- Storage Temperature:For long term storage -80 °C is recommended, but shorter term storage at –20 °C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at –20 °C.
- Shipping Temperature:4 °C
- Immunogen:Dynamin (Ser774) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
- Purification:Affinity Purified
- Cat. No.:10075-438
- Supplier no.:XPS-1006
Specifications
About this item
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.
Affinity Purified
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Aliquot and store at -80°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Dnm1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Sheep
Isotype:
Reactivity: Human, Mouse, Rat