Specifications
- Antibody Type:Primary
- Antigen Name:protein phosphatase 1, regulatory (inhibitor) subunit 1B
- Antigen Symbol:Ppp1r1b
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- Reactivity:Human,Mouse
- Western Blot:Yes
- Size:0.1 mL
- Form:Liquid
- Gene ID:Q6J4I0
- Antigen Synonyms:Ppp1r1b
- Modification:Thr 75
- Storage Buffer:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
- Molecular Weight:32
- Storage Temperature:For long term storage -80 °C is recommended, but shorter term storage at –20 °C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at –20 °C.
- Shipping Temperature:4 °C
- Immunogen:DARPP-32 (Thr75) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse.
- Purification:Affinity Purified
- Cat. No.:10075-436
- Supplier no.:XPS-1005
Specifications
About this item
DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr75) recognizes human and mouse forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Affinity Purified
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Aliquot and store at -80°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Ppp1r1b
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse