Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Pack type:Bottle
- Description:Col-Aurion Total Protein Stain
- Size:50 ml
- Cat. No.:100487-932
- Supplier no.:25536
Specifications
About this item
Col-Aurion is a total protein stain consisting of a solution of coated gold particles with an average particle diameter of 15 nm. The staining principle is based on the electrostatic binding of negatively charged gold particles to proteins with a positive charge present at low pH.
- pH 3.2
- Ready to use
Col-Aurion is developed for the sensitive staining of electrophoretically separated protein bands on nitrocellulose or PVDF™ blotting membranes. Colloidal gold particles accumulate at the site of the protein bands on the membrane, generating an intense dark red staining pattern. The total protein stain assists in assessing immunoblotting results and to evaluate the effectivity of the blotting procedure.
The negative surface charge of colloidal gold particles is responsible for their high affinity for positively charged macromolecules. This characteristic was the basis for the development of a total protein stain based on colloidal gold.
A unique feature of Col-Aurion is the use of BSA-c™ to shield off the surface of the gold particles. Destabilization of gold particles that might occur as a result of interaction with detached protein is thus prevented. The strong negative charge of BSA-c™ gives an additional increase in sensitivity of the stain.