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- Source:Arthrobacter luteus
- Size:1 g
- Storage Conditions:Store at 2...8 °C in the lyophilized state. Approximately 70% of activity is lost when stored at 30 °C for 3 months.
- Enzyme Name:Zymolyase
- Enzyme Activity:20000 units/g
- pH:Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells
pH 6.5, 45 °C for hydrolysis of yeast glucan
pH Range: Stable through pH ranges 5 - 10 - Tested Applications:Used to lyse cell wall of yeast.
- Cat. No.:IC320921
- Supplier no.:320921
Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells.
- Lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate
- Contains 20,000 units enzyme/g
- Primary enzyme responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase
- Hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product
Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells
The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwannimomyces and others.
Activators: Dithiothreitol, 2-mercaptoethanol and other sulfohydryl compounds (as cystein); Essential Enzyme: β-1,3-glucan laminaripentaohydrolase; Other Enzymes: β-1,3-glucanase, ca. 1.5 x 106 units/g, Protease, ca. 1.0 x 104 units/g, Mannanase, ca. 1.0 x 106 units/g, Amylase, Xylanase, Phosphatase, trace.
To sterilize use a non-nitrocellulose filter.
It may also be used for glucan hydrolysis.