Universal miRNA Cloning Linker, New England Biolabs
Catalog # 101418-200
Supplier: New England Biolabs (NEB)
Small Business Enterprise
CAS Number:
Specifications
- Description:Universal miRNA Cloning Linker
- Size:5 ug
- Cat. No.:101418-200
- Supplier no.:S1315S
Specifications
About this item
This 5´ adenylated, 3´ blocked oligodeoxynucleotide can be used for cloning short RNAs according to the procedure of Bartel
RNA ligase recognizes the "activated" adenylated oligo and covalently ligates its 5´ end to the 3´ OH of a second single stranded sequence in the absence of ATP. In a mixture of nucleic acids use of the 5´ adenylated, 3´ blocked oligo with RNA ligase (w/o ATP) results in ligation of the target oligo only.