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Universal miRNA Cloning Linker, New England Biolabs
Catalog # 101418-200
Small Business Enterprise
CAS Number:  
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Universal miRNA Cloning Linker, New England Biolabs
Catalog # 101418-200
Supplier Number:  S1315S
Small Business Enterprise
CAS Number:  

Specifications

  • Description:
    Universal miRNA Cloning Linker
  • Size:
    5 ug
  • Cat. No.:
    101418-200
  • Supplier no.:
    S1315S

Specifications

About this item

This 5´ adenylated, 3´ blocked oligodeoxynucleotide can be used for cloning short RNAs according to the procedure of Bartel

RNA ligase recognizes the "activated" adenylated oligo and covalently ligates its 5´ end to the 3´ OH of a second single stranded sequence in the absence of ATP. In a mixture of nucleic acids use of the 5´ adenylated, 3´ blocked oligo with RNA ligase (w/o ATP) results in ligation of the target oligo only.