NEBNEXT® Poly(A) mRNA Magnetic Isolation Module, New England Biolabs
Supplier: New England Biolabs (NEB)
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About this item
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA.
The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
Specifications
- Method / Format:Magnetic beads
- Prep size:Scalable
- Refine target molecule:poly(A) RNA
- Sample type:Cultured cellsHuman/animal cells and tissues
- Target molecule:RNA
- Description:NEBNext Poly(A) mRNA Magnetic Isolation Module