Blocking of the 3´ -hydroxyl of m7G with 3´-0-Me assures that the capped transcripts are homogeneous

• Reliable expression
• Substantial yields (up to 100 mg/L) in more than 75% of the cases tested
• Expression in either the cytoplasm or periplasm
• Periplasmic expression enhances folding of proteins with disulfide bonds
• Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli
• Gentle elution with maltose
• No detergents or harsh denaturants

The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation.