Mono Q™ columns are strong anion exchange chromatography columns for protein analysis or small scale, high resolution polishing of proteins.
- Packed with MonoBeads™ Q strong anion exchange chromatography resin
- Monodisperse 10 µm porous beads provide high resolution, reproducibility, and durability
- Tricorn column format, compatible with ÄKTA™ systems and other high-performance liquid chromatography (LC) systems
- Greater loading capacity but slightly lower resolution than with MiniBeads columns
Mono Q™ columns are prepacked with MonoBeads™ Q resin, a strong anion exchanger for high-performance ion exchange chromatography (IEX) of proteins, peptides, polynucleotides, and other biomolecules.
MonoBeads™ is a polystyrene/divinyl benzene resin. High efficiency is obtained from the use of small, spherical, monodispersed beads (10 µm), expertly packed in high-performance columns. This high efficiency, coupled with the excellent selectivity of the Q substituents, results in high-resolution separation.
Mono Q™ columns have many applications, including:
• Purification of an RNAi effector nuclease from cultured Drosophila cells; identified as Argonaute2
• Purification and fractionation of whey to study a casein kinase
• Purification of RNF8 prior to evaluating its function as a ubiquitin ligase