About this item
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
- High binding capacity, approx. 40 mg/ml medium
- Negligible leakage of Ni²⁺
- Prepacked columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and additives
This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications. The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
- High binding capacity, approx. 40 mg/ml medium
- Negligible leakage of Ni²⁺
- Prepacked columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and additives
This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications. The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
Documents
Certificates
Search by Lot Number17525501_DATA FILE 1.pdf 17525501_DATA FILE.pdf 17531901_DATA FILE 1.pdf 17531901_DATA FILE 2.pdf 17531901_DATA FILE 3.pdf
Datasheet
17525501_HAND BOOK 1.pdf 17525501_HAND BOOK 2.pdf 17525501_HAND BOOK.pdf 17531901_HAND BOOK 1.pdf 17531901_HAND BOOK 2.pdf
Manual
Cytiva BioXtra Buy-one-get-one US
Promotion Material
Cytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
Specifications for Product Family
Specifications
- Catalog No:
- 95056-022
- 95056-012
- Max. Pressure:
- 5 bar [0.5 MPa] (70 psi)
- 5 bar [0.5 MPa] (70 psi)
- pH Range:
- 3 - 12
- 3 - 12
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifications
Product Family Options
Product Information
- Column hardwareParticle SizeBed dimensionsCapacityFlow rateAvailabilityPrice
- 95056-022HisTrap™ FFSupplier Number: 17-5319-01Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Product available on GSA Advantage®
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Specifications
Cat. No.95056-022Max. Pressure5 bar [0.5 MPa] (70 psi)pH Range3 - 12Storage4 to 30 °C, 20% EthanolProduct available on GSA Advantage® - 95056-012HisTrap™ FFSupplier Number: 17-5255-01Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.95056-012Max. Pressure5 bar [0.5 MPa] (70 psi)pH Range3 - 12Storage4 to 30 °C, 20% Ethanol
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