About this item
Nucleon™ BACC Genomic DNA Extraction Kits were developed for rapid extraction of high quality, high molecular weight genomic DNA from blood and cell cultures.
- Developed for rapid extraction of high-quality, high molecular weight genomic DNA from whole blood and cell cultures
- Recovers DNA ranging in size from 23 to 250 kbp as determined by pulse-field gel electrophoresis
- Features a phenol-free protocol requiring only 30 min to complete
The Nucleon™ proprietary resin is added following cell lysis, deproteinisation with sodium perchlorate, and a single chloroform extraction. Aspiration of the top layer of nucleic acids is simplified and volume of usable sample maximized by a barrier formed between the two layers.
Parameter
Application: Rapid extraction of high-quality, high molecular weight genomic DNA from cell cultures and blood.
Protocol length: 30 min
Sample type: Cell cultures and blood
Yield: 370-440 µg DNA/mg tissue
Mean Purity (A 260/280): 1.8
Nucleon™ BACC1: For 50 preps of 1 ml of whole blood or cultured cells (1 to 3×10⁶ cells/ml)
Nucleon™ BACC3: For 50 preps of 10 ml of whole blood (kit contains all reagents necessary for 50×10 ml blood extractions).
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
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