Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

• A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
• For use in western blotting, dot, and slot blotting applications
• Sensitivity comparable to chemiluminescence
• The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
• Eliminates need for darkroom and film