This kit has been specifically designed for use with RFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs).

Mito-ID® Green Detection Kit contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and aldehyde-fixed-cells

The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes as well as High Content Screening (HCS) platforms. The kit is useful for assessing mitochondrial morphology changes, estimating mitochondrial mass and co-localizing RFP-tagged proteins to the mitochondrial compartment.

Unlike conventional dyes (such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester) Mito-ID® Green dye highlights mitochondria regardless of their energetic state.