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- Description:Fluorescein-12-ddUTP
- Size:25 nmoL
- Purity Detail:Purified by ion-exchange chromatography.
- Purity:≥93% (HPLC)
- Concentration:1mM
- Formulation:Liquid. Solution in water.
- Cat. No.:89165-844
- Supplier no.:42833
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.