Order Entry
Puerto Rico
ContactUsLinkComponent
 
BIOARRAY™ Single-round RNA Amplification and Biotin Labeling System, Enzo Life Sciences
  89165-744
 :  Enzo Life Sciences
 :  
BIOARRAY™ Single-round RNA Amplification and Biotin Labeling System, Enzo Life Sciences
  89165-744
 :  Enzo Life Sciences
 :  ENZ-42420-10
 :  
Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

  • • State issued document with your organization's Federal Tax ID Number
  • • State issued document with your organization's Resale Tax ID Number
  • • City or County issued Business License
  • • State Department of Health Services License
  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.

 

  • Description:
    RNA Amplification and Biotin Labeling System
  • Size:
    1 Pack
  • Storage Conditions:
    −20 °C
  • Cat. No.:
    89165-744
  • Supplier no.:
    42420-10

 

 

The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 250 ng and as high as 5000 ng.

The Single-Round RNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and purification and in vitro transcription labeling.

The higher yield results from an improved cDNA purification system, now provided with the kit.

 : Control RNA (100 µg/mL) is available separately.
 : Kit contains Box A: 40 µL dNTP Mix (dN), Promoter Primer (P), 20 µL First Strand Buffer (FB), 80 µL DTT (D), 10 µL Reverse Transcriptase (RT), 10 µL RNase Inhibitor (I), 50 µL DNA Polymerase (DP), 10 µL RNase H (RH), 150 µL Second Strand Buffer (SB), 60 µL IVT Reaction Buffer (RB), 60 µL 10X Biotin-Labeled Ribonucleotides (B), 60 µL Enhancer Cocktail (EC), 60 µL Enhancer Cocktail (EC), 30 µL T7 RNA Polymerase (T7) and 2.5 mL Nuclease-free Water (W).
Box B: 4.5 mL cDNA Binding Buffer (BB), 1.2L 5X Wash Solution (WS), 10 cDNA Purification Columns, 10 cDNA Elution Tubes and 10 Collection Tubes.