Human Recombinant Proteasome 19S Rpn10/S5a subunit (GST-tag) (from E. coli), GST Tag
Catalog # 89165-254
Supplier: Enzo Life Sciences
CAS Number:
Specifications
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:100 µg
- Tag sequence:GST Tag
- Protein/Peptide Name:Proteasome 19S Rpn10/S5a subunit (GST-tag)
- Purity:>95% (SDS-PAGE)
- Formulation:Colorless liquid. In 20mM TRIS, pH 7.5, containing 500mM sodium chloride.
- Cat. No.:89165-254
- Supplier no.:UW8465-0100
Specifications
About this item
Produced in E. coli.
A subunit of the 26S proteasome, S5a, recognises and binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Purified recombinant S5a retains the ability to bind multi-ubiquitinylated proteins in isolation, when immobilised on glutathione-Sepharose, and after being eluted from glutathione-Sepharose, run on SDS-PAGE, and blotted onto nitrocellulose. Human S5a sequence, with an N-terminal fusion of glutathione S-transferase (Schistosoma japonicum), was expressed in E. coli with GST tag at amino terminus.