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- Description:Fluor-de-Lys® Developer II concentrate
- Size:1.3 ml
- Environmentally Preferable:
- Concentration:5x concentrate (1.25 ml). After dilution (1x), sufficient for 100 assays (50 µl reaction volume).
- Cat. No.:89160-344
- Supplier no.:KI176-1250
- Sufficient for:100 assays (50 µl reaction volume)
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.
Deacetylation of the FLUOR DE LYS® substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an 'inhibitor stop' will be included in the 1x Developer dilution (e.g. nicotinamide for sirtuins).