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FLUOR DE LYS® HDAC Fluorometric Cellular Activity Assay Kit, Enzo Life Sciences
FLUOR DE LYS® HDAC Fluorometric Cellular Activity Assay Kit, Enzo Life Sciences
Catalog # 89156-506
Supplier:  Enzo Life Sciences
CAS Number:  
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FLUOR DE LYS® HDAC Fluorometric Cellular Activity Assay Kit, Enzo Life Sciences
Catalog # 89156-506
Supplier:  Enzo Life Sciences
Supplier Number:  BML-AK503-0001
CAS Number:  

Specifications

  • Size:
    96 Wells
  • Storage Conditions:
    –80 °C (Long Term)
  • Application:
    Fluorescent Detection, HTS Activity Assay, Cell-Based Assays
  • Cat. No.:
    89156-506
  • Supplier no.:
    AK503-0001

Specifications

About this item

The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation

The Fluor-de-Lys® HDAC substrate is cell permeable. It is deacetylated by cellular HDACs and thus suitable for high-throughput cell-based deacetylase assays. This kit provides the reagents and protocols needed for determining rates of intracellular deacetylase activity with cultured cells. Deacetylation can be quantitated by addition of developer to the media and lysed cells.

It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity

Delivery information: Kit includes Nuclear Extracts from Hela Cells (human cervical cancer cell line), FLUOR DE LYS® Substrate (50 µL), 20x FLUOR DE LYS® Developer Concentrate (300 µL), Trichostatin A (HDAC Inhibitor) (100 µL), Nicotinamide (Sirtuin Inhibitor) (500 µL), FLUOR DE LYS® Deacetylated Standard (30 µL), HDAC Assay Buffer (20 mL), Cell Lysis Buffer (20 mL), 1/2 volume microplates (2).