Human Recombinant Caspase-9 (from E. coli)

About this item

Produced in E. coli. Contains an N-terminal His-tag.

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Specifications

Enzyme type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
–80 °C
Form:
Lyophilized
Reconstitution Instructions:
Reconstitute to 1 U/µl with 25 µl or 100 µl PBS containing 15% glycerol.
Note: It is normal to see some insoluble protein following reconstitution. This is mainly due to denaturation of small amounts of protein during preparation. Precipitate should not affe
Enzyme Name:
Caspase-9
Enzyme Activity:
~400 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1 nmol of the caspase substrate LEHD-pNA per hour at 37 °C in a reaction solution containing 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
Enzyme Synonyms:
ICE-LAP-6
Mch6
Purity:
≥90% (SDS-PAGE)