About this item
Profinity eXact Purification Resin:
The Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. Purity of the eluted protein using this purification resin is typically higher than that for other affinity-tag systems. This is due to the specific recognition of subtilisin for its prodomain sequence. The resin can be packed into different column formats, including low- to medium-pressure columns, gravity-flow columns, and mini spin columns, offering users added purification flexibility.
Additional advantages of Profinity eXact purification resin include:
* Purification and processing of fusion-tagged proteins in a single-step
* On-column cleavage in as little as 30 min
* No protease addition required
* Precise cleavage at N-terminus to generate native protein sequence
The resin is supplied in 100 mM sodium phosphate, pH 7.2, containing 0.02% sodium azide. It is also available prepacked in 1 mL and 5 mL cartridges, and in mini spin columns.
The Profinity eXact expression and purification starter kit can be used to easily evaluate Profinity eXact fusion-tag technology and is suitable for new purifications requiring tag removal and for purifications where cleavage has resulted in inferior results. The Profinity eXact expression and purification starter kit includes two kits: the Profinity eXact cloning and expression starter kit for the cloning and expression of a target gene using pPAL vectors and competent cells, and the Profinity eXact mini spin purification starter kit for single-step purification and on-column cleavage of the tagged protein.
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