iScript RT-qPCR Sample Preparation Reagent, Bio-Rad
About this item
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.
* Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
Key Features and Benefits:
* Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
* Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
* Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
* Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
* Use with different cell types — compatible with both suspension and adherent cell cultures
Packaging: Contains RNase inhibitors and RNA stabilizers
2 Options Available Below
Product Details & Documents
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.
* Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
Key Features and Benefits:
* Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
* Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
* Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
* Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
* Use with different cell types — compatible with both suspension and adherent cell cultures
Packaging: Contains RNase inhibitors and RNA stabilizers
Documents
Certificates
Search by Lot NumberProduct Family Options
Product Information
- SizeVolumeAvailabilityPrice
- 101645-420iScript RT-qPCR Sample Preparation ReagentiScript RT-qPCR Sample Preparation Reagent500 reactions5× 10 mlLog in for availabilityEach$1,221.54Supplier Number: 1708899Specifications:MarketsourceMarketsource
- 101645-418iScript RT-qPCR Sample Preparation ReagentSupplier Number: 1708898Specifications:MarketsourceMarketsource
Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.