You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
HIGHDEF® DAB Chromogen/Substrate Set, Enzo Life Sciences
Supplier: Enzo Life Sciences
Used in conjunction with peroxidase-based immunostaining systems.
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Nucleic acid labelling and Detection System, Amersham ECL Direct™
Supplier: Cytiva
ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.
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Caspase-3 Substrate II, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic caspase-3 substrate
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Nucleic acid labelling and detection system, AlkPhos Direct™
Supplier: Cytiva
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
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Enzyme Substrates, Rockland Immunochemical
Supplier: Rockland Immunochemical
(2,2'-Azino-bis[3-ethylbenzthiazoline -6-sulfonic acid]) 1.46 mM A safe, convenient and good, overall performing substrate for ELISA. ABTS develops an intense blue-green color measurable, between 405 nm and 410 nm. Suitable for kinetic and endpoint reactions.
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Amersham ECL Direct Labeling and Detection System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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Saponin Permeating Solution, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Saponin permeating solution, is used as a cell permeabilizing agent.
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Granzyme B Substrate II, Fluorogenic, Calbiochem®, Millipore®
Supplier: MilliporeSigma
A fluorogenic substrate for the detection of granzyme B and caspase-8 activity
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Granzyme B Substrate I, Colorimetric, Calbiochem®, MilliporeSigma
Supplier: MilliporeSigma
Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405nm.
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AttoPhos® AP Fluorescent Substrate System, Promega
Supplier: Promega Corporation
A highly sensitive, flluorescent alkaline phosphatase substrate.
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Proteasome Substrate II, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic proteasome substrate.
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Caspase-9 Substrate I, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6)
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Caspase-1 Substrate X, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
A fluorogenic substrate for detecting caspase-1 (ICE), caspase-4, and caspase-5 activity
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Caspase-1 Substrate VI, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic caspase-1 substrate
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Caspase-3 Substrate VII, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic caspase-3 substrate
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Radiance ECL, Azure Biosystems
Supplier: Azure Biosystems
Radiance ECL is an HRP substrate with long lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other ECL substrates.
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Proteasome Substrate III, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic proteasome substrate that also acts as a substrate for calpain, chymotrypsin, and ingensin.
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Caspase-6 Substrate III, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
A fluorogenic substrate for caspase-6 (Mch2)
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Caspase-3 Substrate IV, Fluorogenic, Calbiochem®
Supplier: MilliporeSigma
Fluorogenic caspase-3 substrate
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Azure Aqua Quad Mini-Cell
Supplier: Azure Biosystems
Compatible with mini gels cassette dimensions 10×8 cm.
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PAGE-Perfect™ Electrophoresis Clean-Up Kit, G-Biosciences
Supplier: G-Biosciences
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis
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Amplite® West ECL HRP Substrate *Femto Sensitivity*
Supplier: AAT BIOQUEST INC
Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.
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4CN Membrane Peroxidase Substrate, KPL
Supplier: KPL
4CN (4-chloro-1-napthol) is a reliable substrate for detection of membrane-bound proteins
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Perfect-FOCUS™ Electrophoresis Clean-Up Kit, G-Biosciences
Supplier: G-Biosciences
Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis
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Synergel™ Agarose Additive, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
Improving Agarose Gel Performance
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SureBlue Reserve™ Microwell Substrate, KPL
Supplier: KPL
SureBlue Reserve™ provides maximum sensitivity for detection of peroxidase labeled conjugates in a microwell format and provides 50% more sensitivity than standard SureBlue™ TMB substrate
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FLASHBlot™ Transfer Buffers, Advansta
Supplier: Advansta
Increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
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Advansta ELISABright™ Chemiluminescent Substrate for ELISA Applications
Supplier: Advansta
ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal-to-noise ratio. ELISABright allows for enhanced detection of low abundance proteins and reduced consumption of antibodies
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Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.
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BioFX® DAB Membrane Substrate, Surmodics
Supplier: Surmodics
DAB membrane substrate is supplied as a two component liquid substrate system designed for the rapid detection of peroxidase in membrane applications.