You searched for: Western Blotting Reagents

Supplier: ABCAM INC CA

Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye

Supplier: ABCAM INC CA

The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

Supplier: ABCAM INC CA

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

Supplier: Cytiva

Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.

Supplier: ABCAM INC CA

Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: Enzo Life Sciences

Species Independent

Supplier: Enzo Life Sciences

Used in conjunction with peroxidase-based immunostaining systems.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Invitrogen

Thermo Scientific Pierce Firefly Signal Enhancer is designed to complement the Pierce Firefly Luciferase Flash and Glow Assay and is recommended when maximum sensitivity is desired.

Supplier: Invitrogen

The background eliminator reduces background signal evenly over any film within minutes

Supplier: Abcam

The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.

Supplier: Invitrogen

Western blot signal enhancer is used to pre-treat either nitrocellulose or PVDF membranes for a 3 – 10X increase in signal intensity for most target proteins.

Supplier: ABCAM INC CA

Antibody Diluent (HAMA Blocker) for ELISA AB318129 is used for sandwich antibody pair based assays that are susceptible to heterophilic antibody interference.

Supplier: ABCAM INC CA

DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection. - Cited in over 375 publications - Stable for up to 6 hours after mixing - Produces crisp brown stain, alcohol-insoluble - Convenient two-component liquid format - Refrigerator-stable for extended usability

Supplier: Abcam

Universal antibody diluent is a PBS formulation at pH 7.3 with protein carrier and preservative that has been developed for stability during long-term storage and for improved antibody performance.

Supplier: Rockland Immunochemical

Enzyme Substrates, Rockland Immunochemical

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: ABCAM INC CA

Antibody Diluent 4BR (ab309955) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: MilliporeSigma

Fluorogenic caspase-3 substrate

Supplier: Cytiva

ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.

Supplier: ABCAM INC CA

High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.

Supplier: ABCAM INC CA

Avidin/biotin blocking kit blocks signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection. - Cited in over 35 publications - Ideal for tissues rich in biotin (e.g., liver, kidney) - Easy-to-use, quick protocol

Supplier: MilliporeSigma

Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405nm.

Supplier: MilliporeSigma

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Supplier: MilliporeSigma

Fluorogenic caspase-1 substrate

Supplier: MilliporeSigma

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6)