Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: MP Biomedicals

Each EtBr Green BagTM contains a standard amount of activated material that will safely and easily concentrate Ethidium Bromide from large liquid quantities

Supplier: Rockland Immunochemical

Opal Prestained Protein Standard 10-180kDa

Supplier: Rockland Immunochemical

Opal Prestained Protein Standard 10-245kDa

Supplier: Azure Biosystems

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and Western blotting transfer modules. The power supply can create and save protocols for all your gel and blotting experiments, and includes a pause/resume function for flexibility.

Supplier: G-Biosciences

Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis

Supplier: G-Biosciences

Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis

Supplier: Lonza

During electrophoresis, these markers help monitor the efficiency of protein separation and allow accurate sizing of proteins in a sample. During Western blotting, they help confirm transfer has occurred from the gel to the membrane.

Supplier: VWR

Bags safely remove ethidium bromide from solution during overnight treatment

Supplier: VWR

VisiGlo HRP and VisiGlo Plus HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates.

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: Azure Biosystems

Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

Supplier: VWR

RiboReady™ Color Micro RNA Ladder is made for precise sizing of RNA molecules in denaturing acrylamide gels

Supplier: VWR

13 fragments ranging from 100 to 3000 bp for easy band identification.

Supplier: VWR

A pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualization dye that can be applied directly to the gel.

Supplier: VWR

8 fragments ranging from 50 to 2000 bp for easy band identification

Supplier: Azure Biosystems

Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

Supplier: Azure Biosystems

Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

Supplier: VWR

10 fragments ranging from 50 to 500 bp for easy band identification

Supplier: VWR

10 fragments ranging from 1,503 to 48,502 bp for easy band identification

Supplier: VWR

11 fragments ranging from 500 to 10,000 bp for easy band identification

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Supplier: Azure Biosystems

Azure Blot Washing Buffer can be used with any Western blotting system.

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

Supplier: Enzo Life Sciences

Species Independent

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.