You searched for: Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.
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CHO/K1 Whole Cell Lysate, Rockland Immunochemical
Supplier: Rockland Immunochemical
Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
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Lysing Matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used for breaking tough plant and animal samples.
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SMART Digest™ Protein Digests Kits
Supplier: Thermo Scientific
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.
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NP-40 Alternative, MilliporeSigma
Supplier: MilliporeSigma
NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.
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PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma
Supplier: MilliporeSigma
A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.
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Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Pronase Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.
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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
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ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega
Supplier: Promega Corporation
ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.
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Trypsin/Lys-C Mix, MS Grade, Promega
Supplier: Promega Corporation
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate
Supplier: Immuno4 LLC
Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios
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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.
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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
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Q-sep® QuEChERS Extraction Salts, Restek
Supplier: Restek
QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
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Factor Xa Protease, New England Biolabs
Supplier: New England Biolabs (NEB)
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg
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Protein Reducing Agents, G-Biosciences
Supplier: G-Biosciences
A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Pierce™ Detergent Removal Spin Column, Thermo Scientific
Supplier: Invitrogen
Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.
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VersaLyse™ RBC Lysing Solution, Beckman Coulter
Supplier: Beckman Coulter
VersaLyse lysing solution is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis.
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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Supplier: Invitrogen
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling
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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific
Supplier: Invitrogen
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.