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Protein Extraction Reagents

Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.

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Pierce™ Micrococcal Nuclease Solution, Thermo Scientific

Pierce™ Micrococcal Nuclease Solution, Thermo Scientific

Supplier: Invitrogen

The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

Supplier: Bio-Rad

A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)

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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Invitrogen

Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Supplier: Invitrogen

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific

Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific

Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific

Supplier: Invitrogen

Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Supplier: Invitrogen

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

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